| Progesterone concentrations in milk can be used to monitor the onset of puberty, postpartum ovarian activity, oestrous cycles and pregnancy in domestic animals.
Assay features;
antibody (monoclonal anti-progesterone) coated solid-phase (96 well plate);
enzyme (peroxidase) conjugate as label;
colour developed using TMB as substrate and read at 450 nm in elisa reader;
standard range from 1 nmol/L to 40 nmol/L
Specificity of antibody:relative to progesterone as 100%
5β-pregnane-3,20-dione
|
4 |
4 |
|
| 5α-pregnane-3,20-dione |
1 |
1 |
|
| 11-deoxycorticosterone |
|
2 |
|
| corticosterone |
|
0 |
.3 |
| pregnenolone |
|
0 |
.3 |
testosterone,cortisol,estriol,
estradiol,estrone all |
< |
0 |
.02 |
125 Iodinated reagents also available.
|
